2R61
Crystal structure of the Staphylococcal superantigen-like protein SSL5 in complex with sialyl-Lewis X at pH 7.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 52.223, 52.223, 236.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.070 - 2.750 |
| R-factor | 0.21216 |
| Rwork | 0.209 |
| R-free | 0.26859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m4v |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.744 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.164 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.129 | 0.484 |
| Total number of observations | 10136 | |
| Number of reflections | 9300 | |
| <I/σ(I)> | 5 | 1.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 20% PEG 3340, 0.2M disodium tartrate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
