2R5Y
Structure of Scr/Exd complex bound to a consensus Hox-Exd site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-03-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 65.310, 65.310, 200.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 11.960 - 2.600 |
R-factor | 0.255 |
Rwork | 0.255 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b8i |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.099 | 0.356 |
Number of reflections | 13550 | |
<I/σ(I)> | 36 | 9.7 |
Completeness [%] | 97.8 | 100 |
Redundancy | 23.2 | 1.86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 277 | 20% MPD, 10-14% PEG 4000, 0.2M sodium acetate, 0.2M potassium chloride, 0.1M Tris , pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
10 | 1 | 2 | Tris | ||
2 | 1 | 2 | MPD | ||
3 | 1 | 1 | PEG 4000 | ||
4 | 1 | 2 | PEG 4000 | ||
5 | 1 | 1 | sodium acetate | ||
6 | 1 | 2 | sodium acetate | ||
7 | 1 | 1 | potassium chloride | ||
8 | 1 | 2 | potassium chloride | ||
9 | 1 | 1 | Tris |