2R5W
Crystal structure of a bifunctional NMN adenylyltransferase/ADP ribose pyrophosphatase from Francisella tularensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 112 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-08 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 124.909, 163.152, 180.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.530 - 2.300 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 40445 | |
| <I/σ(I)> | 29.3 | 2.7 |
| Completeness [%] | 97.9 | 82.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 0.1 M Tris, 0.2 M MgCl2, 19%PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.50 |
