2R5V
Hydroxymandelate Synthase Crystal Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.5957 |
| Spacegroup name | P 41 |
| Unit cell lengths | 106.369, 106.369, 75.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.570 - 2.300 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.949 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.08) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.082 | 0.532 |
| Number of reflections | 38006 | |
| <I/σ(I)> | 15 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 16-20% PEG 3350, 100mM phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
