2R4P
Crystal structure of the long-chain fatty acid transporter FadL mutant G212E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-17 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.510, 69.969, 84.153 |
| Unit cell angles | 90.00, 100.84, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.267 |
| Rwork | 0.267 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t16 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.250 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.088 | 0.449 |
| Number of reflections | 29131 | |
| <I/σ(I)> | 7.8 | 2.3 |
| Completeness [%] | 98.6 | 96.7 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 295 | 18% PEG 8000, 0.2M zinc acetate, 0.1M cacodylate, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
