2R4O
Crystal structure of the long-chain fatty acid transporter FadL mutant delta NPA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-17 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.050, 146.618, 151.227 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.600 |
R-factor | 0.243 |
Rwork | 0.243 |
R-free | 0.31400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t16 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.550 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.540 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.287 | 0.593 |
Number of reflections | 16885 | |
<I/σ(I)> | 3.1 | 2.2 |
Completeness [%] | 98.7 | 97 |
Redundancy | 4.4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 295 | 35% PEG 2000, 0.05M magnesium acetate, 0.05M cacodylate, pH 5.50, VAPOR DIFFUSION, HANGING DROP, temperature 295K |