2R4N
Crystal structure of the long-chain fatty acid transporter FadL mutant N33A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.206, 39.986, 174.996 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.200 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.33200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t16 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.120 | 3.210 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Rmerge | 0.126 | 0.288 |
| Number of reflections | 15295 | |
| <I/σ(I)> | 6.8 | 3.78 |
| Completeness [%] | 85.1 | 76.9 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 295 | 30% PEG 400, 0.1M cadmium chloride, 0.1M sodium acetate, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
