2R4I

CRYSTAL STRUCTURE OF A NTF2-LIKE PROTEIN (CHU_1428) FROM CYTOPHAGA HUTCHINSONII ATCC 33406 AT 1.60 A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-25
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97926, 0.97904
Spacegroup name	P 21 21 21
Unit cell lengths	56.830, 57.690, 157.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.843 - 1.600
R-factor	0.176
Rwork	0.175
R-free	0.19900
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.775
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.843	28.843	1.660
High resolution limit [Å]	1.600	2.730	1.600
Rmerge	0.036	0.023	0.346
Number of reflections	67050
<I/σ(I)>	14.02	29.3	2.3
Completeness [%]	89.2	91.4	82.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	NANODROP, 5.0% Glycerol, 19.0% Isopropanol, 19.0% PEG 4000, 0.1M Citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K