2R4E
Crystal structure of Escherichia coli Glycerol-3-phosphate Dehydrogenase in complex with DHAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Wavelength(s) | 0.9803 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 113.848, 113.822, 193.133 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.20074 |
Rwork | 0.198 |
R-free | 0.25607 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 2.163 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 4.420 | 2.050 |
Rmerge | 0.078 | 0.035 | 0.586 |
Number of reflections | 78152 | ||
<I/σ(I)> | 10.2 | ||
Completeness [%] | 99.5 | 99.5 | 95.9 |
Redundancy | 6 | 6.1 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M di-Ammonium hydrogen phosphate, 0.1M Taps, 12% w/v PEG 6000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |