2R47
Crystal structure of MTH_862 protein of unknown function from Methanothermobacter thermautotrophicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-19 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 150.763, 208.637, 83.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.800 - 1.880 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.22070 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.616 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.800 | 1.930 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.081 | 0.647 |
| Number of reflections | 106479 | |
| <I/σ(I)> | 9.7 | 2.13 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 6.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 2 M Ammonium sulfate, 0.2 M Sodium/potassium tartrate, 0.1 M Sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
