2R3N

Crystal Structure of Cyclin-Dependent Kinase 2 with inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-10-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	53.200, 70.620, 71.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.500 - 1.630
R-factor	0.194
Rwork	0.192
R-free	0.23500
Structure solution method	FOURIER SYNTHESIS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.640
High resolution limit [Å]	1.630	5.570	1.630
Rmerge	0.037	0.014	0.474
Number of reflections	31916
<I/σ(I)>	23
Completeness [%]	92.6	79.6	80.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	298	50 mM Na-HEPES pH 7.4, 50 mM Ammonium Acetate, 8% PEG 4000, 4% Glycerol, 1 mM TCEP, vapor diffusion, temperature 298K