2R3L
Crystal Structure of Cyclin-Dependent Kinase 2 with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.650, 72.580, 72.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.800 - 1.650 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.22600 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
| High resolution limit [Å] | 1.650 | 5.640 | 1.650 |
| Rmerge | 0.045 | 0.019 | 0.319 |
| Number of reflections | 32217 | ||
| <I/σ(I)> | 15.6 | ||
| Completeness [%] | 92.7 | 87.2 | 60.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 298 | 50 mM Na-HEPES pH 7.4, 50 mM Ammonium Acetate, 8% PEG 4000, 4% Glycerol, 1 mM TCEP, vapor diffusion, temperature 298K |
