2R3E
CRYSTAL STRUCTURE OF a ribokinase-like superfamily protein (EF1790) FROM ENTEROCOCCUS FAECALIS V583 AT 1.95 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 4 |
| Unit cell lengths | 109.888, 109.888, 54.193 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.472 - 1.950 |
| R-factor | 0.157 |
| Rwork | 0.155 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r3b |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.427 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.472 | 27.472 | 2.000 |
| High resolution limit [Å] | 1.950 | 8.720 | 1.950 |
| Rmerge | 0.063 | 0.031 | 0.666 |
| Total number of observations | 1142 | 9494 | |
| Number of reflections | 23697 | ||
| <I/σ(I)> | 6.2 | 21.5 | 1.1 |
| Completeness [%] | 99.9 | 88.1 | 100 |
| Redundancy | 5.4 | 4.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | NANODROP, 10.0% PEG 6000, 0.1M Bicine pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
