2R39
Crystal structure of FixG-related protein from Vibrio parahaemolyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-04 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97891 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 74.567, 74.567, 49.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.020 - 2.020 |
R-factor | 0.20513 |
Rwork | 0.203 |
R-free | 0.23815 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.326 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.069 | 0.273 |
Number of reflections | 10403 | |
<I/σ(I)> | 50.53 | 7.25 |
Completeness [%] | 99.6 | 100 |
Redundancy | 10.1 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | HEPES pH 7.5, 30% PEG 400, NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 289K |