Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.510, 61.430, 32.990 |
| Unit cell angles | 90.00, 114.87, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| Rwork | 0.191 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | X-RAY COORDINATES OF BOWMAN-BIRK INHIBITOR FROM ADZUKI BEAN |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEIT |
| Phasing software | AMoRE |
| Refinement software | REFMAC (3.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 4072 | |
| <I/σ(I)> | 13.9 | 5.8 |
| Completeness [%] | 99.0 | 94.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 30% SATURATED AMMONIUM SULFATE 0.1M CITRATE-PHOSPHATE, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
