2R31
Crystal structure of atp12p from paracoccus denitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.553, 55.749, 96.616 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.000 |
| R-factor | 0.1187 |
| Rwork | 0.118 |
| R-free | 0.14250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p4x |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.032 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.060 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.062 | 0.745 |
| Number of reflections | 127208 | |
| <I/σ(I)> | 25.41 | 3.97 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 14.49 | 14.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 278 | 30% PEG4000, 50 MM MGCL2, 100 MM TRIS VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 278K, pH 8.5 |
