2R2Y
Crystal structure of the proteasomal Rpn13 PRU-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.428, 56.223, 63.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.950 - 1.700 |
| R-factor | 0.21453 |
| Rwork | 0.214 |
| R-free | 0.23005 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.429 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.051 | 0.272 |
| Number of reflections | 16853 | |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 99.9 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | ||||
| 1 | ||||
| 1 |
