2R2Q

Crystal structure of human Gamma-Aminobutyric Acid Receptor-Associated Protein-like 1 (GABARAP1), Isoform CRA_a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-08-10
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	36.860, 38.193, 45.746
Unit cell angles	111.09, 95.91, 108.05

Refinement procedure

Resolution	18.500 - 1.650
R-factor	0.201
Rwork	0.200
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.017
RMSD bond angle	1.449
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.710
High resolution limit [Å]	1.650	3.550	1.650
Rmerge	0.042	0.052	0.082
Number of reflections	24587
<I/σ(I)>	27.5
Completeness [%]	94.9	98.7	65
Redundancy	3.5	3.8	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	25% PEG 3350, 0.2M Sodium chloride, 0.1M Hepes, 5% MPD, pH 7.5, VAPOR DIFFUSION, temperature 291K
1	VAPOR DIFFUSION	7.5	291	25% PEG 3350, 0.2M Sodium chloride, 0.1M Hepes, 5% MPD, pH 7.5, VAPOR DIFFUSION, temperature 291K