2R2K
Crystal structure of the complex of camel peptidoglycan recognition protein with disaccharide at 3.2A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2007-07-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 89.272, 102.560, 163.693 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.000 - 3.250 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yck |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.000 | 3.370 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.342 | |
Number of reflections | 11198 | |
<I/σ(I)> | 5.2 | 9 |
Completeness [%] | 92.3 | 95.9 |
Redundancy | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 290 | 0.3M NaKTartrate, 25% PEG, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |