2R2F
RIBONUCLEOTIDE REDUCTASE R2F PROTEIN FROM SALMONELLA TYPHIMURIUM (OXIDIZED)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.490, 71.740, 96.070 |
| Unit cell angles | 90.00, 95.01, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.26800 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.212 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.066 * | 0.253 * |
| Number of reflections | 36792 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 3.3 | 3.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 15 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 50 (mM) | |
| 2 | 1 | drop | dithiothreitol | 10 (mM) | |
| 3 | 1 | drop | protein | 10-30 (mg/ml) | |
| 4 | 1 | reservoir | PEG8000 | 15-25 (%) | |
| 5 | 1 | reservoir | 0.7 (M) | ||
| 6 | 1 | reservoir | Tris |
