2R2C
Crystal structure of a domain of the outer membrane lipoprotein Omp28 from Porphyromonas gingivalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97940 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.831, 110.342, 90.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.630 - 1.800 |
R-factor | 0.17527 |
Rwork | 0.174 |
R-free | 0.20076 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.386 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.630 | 1.848 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.074 | 0.630 |
Number of reflections | 34354 | |
<I/σ(I)> | 37.5 | 2 |
Completeness [%] | 99.1 | 94.46 |
Redundancy | 9.4 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Sodium citrate tribasic dihydrate, 20% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |