2R2C
Crystal structure of a domain of the outer membrane lipoprotein Omp28 from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.831, 110.342, 90.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.630 - 1.800 |
| R-factor | 0.17527 |
| Rwork | 0.174 |
| R-free | 0.20076 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.386 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.630 | 1.848 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.630 |
| Number of reflections | 34354 | |
| <I/σ(I)> | 37.5 | 2 |
| Completeness [%] | 99.1 | 94.46 |
| Redundancy | 9.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Sodium citrate tribasic dihydrate, 20% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
