2R22
Structure of the native RNA tridecamer r(GCGUUUGAAACGC) at 1.5 A (NatMn)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.919487 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.280, 25.500, 29.600 |
| Unit cell angles | 97.97, 106.69, 101.62 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| Rwork | 0.178 |
| R-free | 0.20000 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.892 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.090 | 0.279 |
| Number of reflections | 12814 | |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 88.7 | 92 |
| Redundancy | 1.7 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 3 mM MnCl2, 100 mM NaCl, 50 mM Tris, 30 % MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | Tris | ||
| 4 | 1 | 1 | MnCl2 | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | Tris | ||
| 8 | 1 | 2 | MnCl2 |
