2R21
Structure of the RNA brominated tridecamer r(GCGUU-5BUGAAACGC) at 1.6 A (BrMn)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 80 |
Detector technology | IMAGE PLATE |
Collection date | 2003-05-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.919487,0.919704,0.88558,0.979776 |
Spacegroup name | P 1 |
Unit cell lengths | 25.250, 25.650, 29.720 |
Unit cell angles | 98.13, 106.15, 100.83 |
Refinement procedure
Resolution | 20.000 - 1.590 |
Rwork | 0.169 |
R-free | 0.19100 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.893 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.680 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.117 | 0.230 |
Number of reflections | 8713 | |
<I/σ(I)> | 4.8 | 2.6 |
Completeness [%] | 94.4 | 84.9 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.5mM MnCl2, 100mM NaCl, 30% MPD, 50mM Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | Tris | ||
4 | 1 | 1 | MnCl2 | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | NaCl | ||
7 | 1 | 2 | Tris | ||
8 | 1 | 2 | MnCl2 |