2R20
Structure of the RNA brominated tridecamer r(GCGUU-5BUGAAACGC) at 1.3 A (Br2)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 80 |
Detector technology | IMAGE PLATE |
Collection date | 2002-05-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.919487,0.919704,0.88558,0.979776 |
Spacegroup name | P 1 |
Unit cell lengths | 25.280, 25.800, 29.990 |
Unit cell angles | 98.62, 105.58, 100.92 |
Refinement procedure
Resolution | 20.000 - 1.300 |
Rwork | 0.181 |
R-free | 0.19500 |
Structure solution method | MAD |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.450 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 13060 | |
<I/σ(I)> | 9.1 | 8.6 |
Completeness [%] | 91.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 5 mM MgCl2, 5 mM MnCl2, 100 mM NaCl, 30 % MPD, 50 mM tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
10 | 1 | 2 | MnCl2 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | tris | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 1 | MnCl2 | ||
6 | 1 | 2 | MPD | ||
7 | 1 | 2 | NaCl | ||
8 | 1 | 2 | tris | ||
9 | 1 | 2 | MgCl2 |