2R1U
DJ-1 activation by catechol quinone modification
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-22 |
Spacegroup name | P 31 |
Unit cell lengths | 75.688, 75.688, 75.045 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.680 - 1.500 |
Rwork | 0.185 |
R-free | 0.19500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.316 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.040 | 0.040 |
Number of reflections | 71845 | |
<I/σ(I)> | 40 | 40 |
Completeness [%] | 93.3 | 100 |
Redundancy | 6.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 29%PEG4K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |