2R1D
Crystal structure of rat neurexin 1beta in the Ca2+ containing form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2001-01-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 116.683, 195.719, 103.867 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.20482 |
| Rwork | 0.203 |
| R-free | 0.24401 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1c4r |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.614 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.600 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.065 | 0.423 |
| Number of reflections | 73949 | |
| <I/σ(I)> | 24.8 | 4.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 21% Peg 5000 MME, 0.1 M sodium cacodylate pH 6.5, 0.2 M AmSO4, 1.86% 1,2,3-heptanetriol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
