2R1B
Crystal Structure of rat neurexin 1beta with a splice insert at SS#4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.122, 39.260, 85.410 |
| Unit cell angles | 90.00, 115.47, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.720 |
| R-factor | 0.19956 |
| Rwork | 0.197 |
| R-free | 0.22474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c4r |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.708 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.054 | 0.355 |
| Number of reflections | 49680 | |
| <I/σ(I)> | 25.9 | 3.1 |
| Completeness [%] | 92.1 | 73.1 |
| Redundancy | 8.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 17% Peg 8000, 0.1 M Tris pH 8.5, 0.2 M MgCl2, 5 mM CaCl2, 0.5% beta-octyl-glucoside, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
