2R1A
Crystal structure of the periplasmic lipopolysaccharide transport protein LptA (YhbN), trigonal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.977173 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 146.210, 146.210, 186.810 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.980 - 3.260 |
R-factor | 0.3011 |
Rwork | 0.298 |
R-free | 0.36086 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r19 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.035 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0065) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.370 |
High resolution limit [Å] | 3.250 | 7.000 | 3.250 |
Rmerge | 0.127 | 0.050 | 0.614 |
Number of reflections | 30606 | ||
<I/σ(I)> | 8.3 | ||
Completeness [%] | 97.3 | 98.4 | 81.8 |
Redundancy | 8.9 | 9.5 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | PEG 3350, Glycerol, LPS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |