2R19
Crystal structure of the periplasmic lipopolysaccharide transport protein LptA (YhbN), orthorhombic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.10000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.910, 58.410, 149.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.030 - 2.160 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.27100 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.611 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
Rmerge | 0.116 | 0.031 | 0.739 |
Number of reflections | 13258 | ||
<I/σ(I)> | 6.5 | ||
Completeness [%] | 99.6 | 97.6 | 100 |
Redundancy | 6.4 | 6.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 3350, Glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 3350, Glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |