2R16
Crystal Structure of bovine neurexin 1 alpha LNS/LG domain 4 (with no splice insert)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2006-08-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.96112 |
Spacegroup name | P 63 |
Unit cell lengths | 90.322, 90.322, 39.624 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.700 - 1.040 |
R-factor | 0.14839 |
Rwork | 0.146 |
R-free | 0.16518 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c4r |
RMSD bond length | 0.016 |
RMSD bond angle | 1.906 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.700 | 1.080 |
High resolution limit [Å] | 1.040 | 1.040 |
Rmerge | 0.070 | 0.296 |
Number of reflections | 82197 | |
<I/σ(I)> | 37.9 | 6.2 |
Completeness [%] | 92.9 | 60.5 |
Redundancy | 12.6 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 12.5% isopropanol, 0.1 M CHES pH 9.0, 5 mM CaCl2, , VAPOR DIFFUSION, HANGING DROP, temperature 293K |