2R15

Crystal structure of the myomesin domains 12 and 13

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-14
Detector	MAR CCD 165 mm
Wavelength(s)	0.8126, 0.91837, 0.8126
Spacegroup name	C 2 2 21
Unit cell lengths	61.504, 87.870, 203.081
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.870 - 2.240
Rwork	0.196
R-free	0.22300
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.380
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.240	2.240
Rmerge	0.076	0.382
Number of reflections	25757
<I/σ(I)>	9.9
Completeness [%]	95.7	93.2
Redundancy	4.9	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	0.16M CH3COONH4, 14% (W/V) PEG 20000, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	0.16M CH3COONH4, 14% (W/V) PEG 20000, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 292K