2R13
Crystal structure of human mitoNEET reveals a novel [2Fe-2S] cluster coordination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 58.794, 58.794, 175.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.16037 |
| Rwork | 0.159 |
| R-free | 0.18796 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.491 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.073 | 0.440 |
| Number of reflections | 14636 | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 9.1 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.0M NaCl, 0.1M Tris-HCl pH5.5-9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
