2R0Y

Structure of the Rsc4 tandem bromodomain in complex with an acetylated H3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	H 3 2
Unit cell lengths	95.028, 95.028, 233.077
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.750
R-factor	0.186
Rwork	0.185
R-free	0.21900
RMSD bond length	0.016
RMSD bond angle	1.406
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.750	3.770	1.750
Rmerge	0.068	0.047	0.597
Number of reflections	39000
<I/σ(I)>	17.3
Completeness [%]	94.6	95.1	72.4
Redundancy	4.2	4.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.2	298	0.1M phosphate-citrate, 0.2M NaCl, 0.2M ammonium sulfate, 20% PEG 3000, 5% Glycerol, pH 4.2, vapor diffusion, temperature 298K