2R0Y
Structure of the Rsc4 tandem bromodomain in complex with an acetylated H3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 95.028, 95.028, 233.077 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 1.750 |
R-factor | 0.186 |
Rwork | 0.185 |
R-free | 0.21900 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.406 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
Rmerge | 0.068 | 0.047 | 0.597 |
Number of reflections | 39000 | ||
<I/σ(I)> | 17.3 | ||
Completeness [%] | 94.6 | 95.1 | 72.4 |
Redundancy | 4.2 | 4.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.2 | 298 | 0.1M phosphate-citrate, 0.2M NaCl, 0.2M ammonium sulfate, 20% PEG 3000, 5% Glycerol, pH 4.2, vapor diffusion, temperature 298K |