2R0X

Crystal structure of a putative flavin reductase (ycdh, hs_1225) from haemophilus somnus 129pt at 1.06 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97925, 0.97898
Spacegroup name	C 1 2 1
Unit cell lengths	72.020, 38.850, 48.440
Unit cell angles	90.00, 115.63, 90.00

Refinement procedure

Resolution	23.402 - 1.060
R-factor	0.143
Rwork	0.141
R-free	0.17000
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.667
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.402	23.402	1.100
High resolution limit [Å]	1.060	1.810	1.060
Rmerge	0.070	0.044	0.678
Number of reflections	54635
<I/σ(I)>	6.34	14.3	1.34
Completeness [%]	98.9	98.3	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	NANODROP, 2.0M Ammonium sulfate, 0.1M Sodium acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K