2R0S
Crystal Structure of the Rsc4 tandem bromodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 95.899, 95.899, 233.519 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21800 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.243 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.369 |
| Number of reflections | 37132 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 96.2 | 67.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.2 | 298 | 0.1M phosphate-citrate, 0.2M NaCl, 0.2M ammonium sulfate, 20% PEG 3000, 5% Glycerol, pH 4.2, VAPOR DIFFUSION, temperature 298K |
