2R0J
Crystal structure of the putative ubiquitin conjugating enzyme, PFE1350c, from Plasmodium falciparum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-06-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.747, 73.747, 79.112 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.990 - 1.850 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qcq |
RMSD bond length | 0.033 |
RMSD bond angle | 2.313 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.047 | 0.567 |
Number of reflections | 19224 | |
<I/σ(I)> | 20.6 | 1.6 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 13.8 | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 3.5M Sodium formate, 0.1M Bis-Tris pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |