2R0H
Fungal lectin CGL3 in complex with chitotriose (chitotetraose)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.900, 59.700, 60.200 |
| Unit cell angles | 73.00, 90.70, 76.40 |
Refinement procedure
| Resolution | 14.920 - 1.900 |
| R-factor | 0.19791 |
| Rwork | 0.195 |
| R-free | 0.25374 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uld |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.133 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.057 | 0.171 |
| Number of reflections | 56130 | |
| <I/σ(I)> | 11.59 | 7.32 |
| Completeness [%] | 82.5 | 49.9 |
| Redundancy | 2.5 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.1M sodium citrate, 0.9M lithium sulfate, 0.5M ammonium sulfate, pH5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
