2R0H
Fungal lectin CGL3 in complex with chitotriose (chitotetraose)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.966 |
Spacegroup name | P 1 |
Unit cell lengths | 47.900, 59.700, 60.200 |
Unit cell angles | 73.00, 90.70, 76.40 |
Refinement procedure
Resolution | 14.920 - 1.900 |
R-factor | 0.19791 |
Rwork | 0.195 |
R-free | 0.25374 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uld |
RMSD bond length | 0.013 |
RMSD bond angle | 2.133 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.057 | 0.171 |
Number of reflections | 56130 | |
<I/σ(I)> | 11.59 | 7.32 |
Completeness [%] | 82.5 | 49.9 |
Redundancy | 2.5 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.1M sodium citrate, 0.9M lithium sulfate, 0.5M ammonium sulfate, pH5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |