2R0F
Ligand free structure of fungal lectin CGL3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 112.375, 112.375, 175.664 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.840 - 2.000 |
| R-factor | 0.24301 |
| Rwork | 0.243 |
| R-free | 0.24949 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uld |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.404 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.163 | |
| Number of reflections | 41642 | |
| <I/σ(I)> | 9.8 | 8 |
| Completeness [%] | 91.0 | 83.2 |
| Redundancy | 4.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 291 | MPD, sodium citrate, pH4.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
