2R0D

Crystal Structure of Autoinhibited Form of Grp1 Arf GTPase Exchange Factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	82.906, 94.705, 115.436
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.040
R-factor	0.20962
Rwork	0.208
R-free	0.24162
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.084
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900
Number of reflections	58263

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	277.15	10% PEG 4000, 50 mM Tris, 50 mM LiSO4, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K