2R0B
Crystal structure of human tyrosine phosphatase-like serine/threonine/tyrosine-interacting protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.932, 53.358, 60.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.262 |
| Rwork | 0.260 |
| R-free | 0.28300 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.395 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.011 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.132 | 0.343 |
| Number of reflections | 21898 | |
| <I/σ(I)> | 8.6 | 3.9 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM Bis-Tris pH 6.5, 1.8M Ammonium sulfate, VAPOR DIFFUSION, temperature 294K |
