2R03
Crystal Structure of ALIX/AIP1 in complex with the YPDL Late Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Wavelength(s) | 0.97946 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.383, 98.570, 72.799 |
Unit cell angles | 90.00, 107.07, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.590 |
R-factor | 0.226 |
Rwork | 0.223 |
R-free | 0.28600 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.384 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.590 | 5.600 | 2.590 |
Rmerge | 0.150 | 0.128 | 0.306 |
Number of reflections | 29646 | ||
<I/σ(I)> | 16.7 | ||
Completeness [%] | 98.2 | 92 | 96.5 |
Redundancy | 3 | 3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 286 | 0.20-0.25 M MgCl2, 7-10% PEG 4000, 0.1 M NaMES, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 286K |