2R02
Crystal Structure of ALIX/AIP1 in complex with the HIV-1 YPLTSL Late Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.923, 99.078, 73.226 |
| Unit cell angles | 90.00, 107.32, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.228 |
| Rwork | 0.224 |
| R-free | 0.28500 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.511 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.060 | 0.037 | 0.252 |
| Number of reflections | 29846 | ||
| <I/σ(I)> | 24 | ||
| Completeness [%] | 98.4 | 92.2 | 94.2 |
| Redundancy | 3.8 | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop vapor diffusion | 5.9 | 286 | 0.20-0.25 M MgCl2, 7-10% PEG 4000, 0.1 M NaMES, pH 5.9, sitting drop vapor diffusion, temperature 286K |
