Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.349, 48.023, 126.317 |
| Unit cell angles | 90.00, 111.39, 90.00 |
Refinement procedure
| Resolution | 35.090 - 1.900 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2faf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.324 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.117 | 0.061 | 0.596 |
| Number of reflections | 95642 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 97.9 | 99.3 | 95.8 |
| Redundancy | 4.9 | 5.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 19% PEG6000, 0.1M HEPES pH 7.4, N-OCTANOYL SUCROSE, hanging drop vapor diffusion, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
