2QZS
Crystal Structure of Wild-type E.coli GS in complex with ADP and Glucose(wtGSb)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 41 |
| Unit cell lengths | 126.494, 126.494, 151.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.720 - 2.200 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rzu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.588 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.057 | 0.289 |
| Number of reflections | 60327 | |
| <I/σ(I)> | 13.3 | 5.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 277 | 40%(w/v) PEG 4000, 0.2 M Na tartrate, 0.1 M HEPPSO, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
