2QZI
The crystal structure of a conserved protein of unknown function from Streptococcus thermophilus LMG 18311.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 150.783, 150.783, 103.247 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.230 - 2.200 |
| R-factor | 0.21229 |
| Rwork | 0.210 |
| R-free | 0.25112 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.961 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.230 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.095 | 0.630 |
| Number of reflections | 35201 | |
| <I/σ(I)> | 54.3 | 4.1 |
| Completeness [%] | 99.2 | 92.7 |
| Redundancy | 18 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 35% Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
