2QZ9
crystal structure of aspartate semialdehyde dehydrogenase II from vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.327, 85.655, 116.101 |
| Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
| Resolution | 37.610 - 2.200 |
| R-factor | 0.20091 |
| Rwork | 0.198 |
| R-free | 0.24978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PEB entry 1YS4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.400 | |
| Number of reflections | 54355 | |
| <I/σ(I)> | 23 | 3 |
| Completeness [%] | 99.6 | 91.4 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 24% PEG8000, 0.2M ammonium sulfate, 5mM DTT, 0.1M sodium citrate pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
