2QZ9
crystal structure of aspartate semialdehyde dehydrogenase II from vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.327, 85.655, 116.101 |
Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
Resolution | 37.610 - 2.200 |
R-factor | 0.20091 |
Rwork | 0.198 |
R-free | 0.24978 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PEB entry 1YS4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.400 | |
Number of reflections | 54355 | |
<I/σ(I)> | 23 | 3 |
Completeness [%] | 99.6 | 91.4 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 24% PEG8000, 0.2M ammonium sulfate, 5mM DTT, 0.1M sodium citrate pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |