2QZ4
Human paraplegin, AAA domain in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 57.200, 57.200, 155.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.130 - 2.220 |
| R-factor | 0.21153 |
| Rwork | 0.209 |
| R-free | 0.26171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ce7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.603 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.069 | 0.203 |
| Number of reflections | 13190 | |
| <I/σ(I)> | 23.7 | 7.2 |
| Completeness [%] | 95.1 | 62.7 |
| Redundancy | 10.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25% PEG 3350, 100mM Bis-Tris pH 6.5, 200mM Ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
