2QZ2
Crystal structure of a glycoside hydrolase family 11 xylanase from Aspergillus niger in complex with xylopentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9918 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 67.326, 67.326, 165.401 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.480 - 2.800 |
| R-factor | 0.22632 |
| Rwork | 0.221 |
| R-free | 0.27678 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ukr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.005 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 5843 | |
| <I/σ(I)> | 13.7 | 5.3 |
| Completeness [%] | 98.2 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.6M ammonium sulphate, 0.1M HEPES pH 7.5, 0.1 M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
