2QYZ
Crystal structure of the uncharacterized protein CTC02137 from Clostridium tetani E88
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 40.711, 40.711, 156.753 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.700 - 2.040 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.25900 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.008 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 4.390 | 2.040 |
| Rmerge | 0.035 | 0.024 | 0.183 |
| Number of reflections | 16582 | ||
| <I/σ(I)> | 18.3 | ||
| Completeness [%] | 89.3 | 96.3 | 39.6 |
| Redundancy | 4.9 | 5.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 294 | 0.2M Ammonium sulfate, 0.1M Bis-Tris pH 5.5, 25% PEG 3350, VAPOR DIFFUSION, temperature 294K |
