2QYW
Crystal structure of mouse vti1b Habc domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.227, 25.167, 80.115 |
| Unit cell angles | 90.00, 95.23, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.22518 |
| Rwork | 0.218 |
| R-free | 0.29653 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.678 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.490 |
| Number of reflections | 6751 | |
| <I/σ(I)> | 26.4 | 4 |
| Completeness [%] | 96.9 | 96.9 |
| Redundancy | 9.9 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 100 mM Tris, 30% ethanol and 20% glycerol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
